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  • 产品名称：Human GATA3 (Trans-acting T-cell-specific transcription factor GATA-3) CLIA Kit (STJC0000441)

  • 产品型号：STJC0000441
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human GATA3 (Trans-acting T-cell-specific transcription factor GATA-3) CLIA Kit (STJC0000441)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human GATA3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: GATA3
Gene ID: 2625
Uniprot ID: GATA3_HUMAN
Specificity: This kit recognizes Human GATA3 in samples. No significant cross-reactivity or interference between Human GATA3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity T-cells and endothelial cells.
Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression. Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.
Protein Name Trans-Acting T-Cell-Specific Transcription Factor Gata-3
Gata-Binding Factor 3
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-6785807
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative CLIA Names Trans-Acting T-Cell-Specific Transcription Factor Gata-3 CLIA kit
Gata-Binding Factor 3 CLIA kit
GATA3 CLIA kit
Specificity This kit recognizes Human GATA3 in samples. No significant cross-reactivity or interference between Human GATA3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 92
EDTA plasma(n=8) 95-108 102
Cell culture media(n=8) 85-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 185.12 568.83 1430.94 186.26 563.60 1531.82
Standard deviation 16.75 61.09 136.37 22.71 65.66 163.75
CV (%) 9.05 10.74 9.53 12.19 11.65 10.69
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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