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  • 产品名称：Human GPC3 (Glypican-3) CLIA Kit (STJC0000758)

  • 产品型号：STJC0000758
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human GPC3 (Glypican-3) CLIA Kit (STJC0000758)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human GPC3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: GPC3
Gene ID: 2719
Uniprot ID: GPC3_HUMAN
Specificity: This kit recognizes Human GPC3 in samples. No significant cross-reactivity or interference between Human GPC3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Detected in placenta (at protein level). Highly expressed in lung, liver and kidney.
Post Translational Modifications O-glycosylated.contains heparan sulfate and/or chondroitin sulfate. Cleaved intracellularly by a furin-like convertase to generate 2 subunits, alpha and beta, which remain associated through disulfide bonds and are associated with the cell surface via the GPI-anchor. This processing is essential for its role in inhibition of hedgehog signaling. A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface.
Function Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation.
Protein Name Glypican-3
Gtr2-2
Intestinal Protein Oci-5
Mxr7 Cleaved Into - Glypican-3 Alpha Subunit - Glypican-3 Beta Subunit
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022928
Reactome: R-HSA-2024096
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Reactome: R-HSA-381426
Reactome: R-HSA-4420332
Reactome: R-HSA-8957275
Reactome: R-HSA-9694614
Reactome: R-HSA-975634
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Alternative CLIA Names Glypican-3 CLIA kit
Gtr2-2 CLIA kit
Intestinal Protein Oci-5 CLIA kit
Mxr7 Cleaved Into - Glypican-3 Alpha Subunit - Glypican-3 Beta Subunit CLIA kit
GPC3 CLIA kit
OCI5 CLIA kit
Specificity This kit recognizes Human GPC3 in samples. No significant cross-reactivity or interference between Human GPC3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-114 107
EDTA plasma(n=8) 90-101 95
Cell culture media(n=8) 100-112 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 109.97 275.43 862.58 111.40 250.53 948.60
Standard deviation 13.86 23.93 63.31 11.53 27.83 108.52
CV (%) 12.60 8.69 7.34 10.35 11.11 11.44
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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