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  • 产品名称：Human HMOX1 (Heme oxygenase 1) Sandwich ELISA (STJE0003507)

  • 产品型号：STJE0003507
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human HMOX1 (Heme oxygenase 1) Sandwich ELISA (STJE0003507)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This HMOX1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: HMOX1
Gene ID: 3162
Uniprot ID: HMOX1_HUMAN
Specificity: This kit recognizes Human HO1 in samples. No significant cross-reactivity or interference between Human HO1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications A soluble form arises by proteolytic removal of the membrane anchor.
Function Heme oxygenase 1: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis. Heme oxygenase 1: (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Protein Name Heme Oxygenase 1
Ho-1 Cleaved Into - Heme Oxygenase 1 Soluble Form
Database Links Reactome: R-HSA-189483
Reactome: R-HSA-6785807
Reactome: R-HSA-844456
Reactome: R-HSA-917937
Reactome: R-HSA-9609523
Reactome: R-HSA-9660826
Reactome: R-HSA-9707564
Reactome: R-HSA-9707587
Reactome: R-HSA-9707616
Reactome: R-HSA-9818027
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Alternative ELISA Names Heme Oxygenase 1 ELISA kit
Ho-1 Cleaved Into - Heme Oxygenase 1 Soluble Form ELISA kit
HMOX1 ELISA kit
HO ELISA kit
HO1 ELISA kit
Specificity This kit recognizes Human HO1 in samples. No significant cross-reactivity or interference between Human HO1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-107 101
EDTA plasma (n=8) 92-110 100
Cell culture media (n=8) 84-97 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.09 3.15 9.57 0.99 3.28 9.54
Standard deviation 0.07 0.18 0.39 0.05 0.15 0.48
CV (%) 6.42 5.71 4.08 5.05 4.57 5.03
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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