产品详情
简单介绍:
Human IAPP (Islet amyloid polypeptide) Competitive ELISA (STJE0002128)
详情介绍:
Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 37.5pg/mL |
Detection Limit: | 62.5~4000pg/mL |
Short Description: | This IAPP Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 2.5h |
Detection: | Colormetric |
Gene Symbol: | IAPP |
Gene ID: | 3375 |
Uniprot ID: | IAPP_HUMAN |
Specificity: | This kit recognizes Human IAPP in samples. No significant cross-reactivity or interference between Human IAPP and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. |
Protein Name | Islet Amyloid Polypeptide Amylin Diabetes-Associated Peptide Dap Insulinoma Amyloid Peptide |
Database Links | Reactome: R-HSA-210745 Reactome: R-HSA-418555 Reactome: R-HSA-419812 Reactome: R-HSA-977225 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Islet Amyloid Polypeptide ELISA kit Amylin ELISA kit Diabetes-Associated Peptide ELISA kit Dap ELISA kit Insulinoma Amyloid Peptide ELISA kit IAPP ELISA kit |
Specificity | This kit recognizes IAPP in samples. No significant cross-reactivity or interference between IAPP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Serum(n=8) | 85-100 | 92 |
EDTA plasma(n=8) | 85-96 | 91 |
Cell culture media(n=8) | 95-107 | 101 |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 193.90 | 485.90 | 1754.10 | 197.00 | 478.40 | 1764.50 |
Standard deviation | 9.90 | 21.90 | 84.20 | 12.60 | 21.00 | 84.70 |
CV (%) | 5.11 | 4.51 | 4.80 | 6.40 | 4.39 | 4.80 |
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