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  • 产品名称：Human IFIH1 (Interferon Induced Helicase C Domain Containing Protein 1) Sandwich ELISA Kit (STJE0009650)

  • 产品型号：STJE0009650
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IFIH1 (Interferon Induced Helicase C Domain Containing Protein 1) Sandwich ELISA Kit (STJE0009650)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.120ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This IFIH1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: IFIH1
Gene ID: 64135
Uniprot ID: IFIH1_HUMAN
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.
Post Translational Modifications Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta. Ubiquitinated by RNF125, leading to its degradation by the proteasome. USP17/UPS17L2-dependent deubiquitination positively regulates the receptor. Ubiquitinated by TRIM25 via 'Lys-63'-linked ubiquitination, promoting activation of IFIH1/MDA5. Ubiquitinated by TRIM40 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. Ubiquitinated by TRIM65 via 'Lys-63'-linked ubiquitination, promoting activation of IFIH1/MDA5. ISGylated by ISG15. ISGylation increases upon infection with dengue (DENV) or Zika (ZIKV) viruses. ISGylation at Lys-23 and Lys-43 is dependent of dephosphorylation at Ser-88, regulates mitochondrial translocation and oligomerization. Essential for IFIH1/MDA5-mediated cytokine responses and restriction of virus replication. Phosphorylated at Ser-88. Dephosphorylated by phsophatases PP1.dephosphorylation precedes and is required for ISGylation. During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation. (Microbial infection) Cleaved and inactivated by the protease 2A of coxsackievirus B3, poliovirus and enterovirus 71 allowing the virus to disrupt the host type I interferon production.
Function Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs).IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus. Detects coronavirus SARS-CoV-2. Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.
Protein Name Interferon-Induced Helicase C Domain-Containing Protein 1
Clinically Amyopathic Dermatomyositis Autoantigen 140 Kda
Cadm-140 Autoantigen
Helicase With 2 Card Domains
Helicard
Interferon-Induced With Helicase C Domain Protein 1
Melanoma Differentiation-Associated Protein 5
Mda-5
Murabutide Down-Regulated Protein
Rig-I-Like Receptor 2
Rlr-2
Rna Helicase-Dead Box Protein 116
Database Links Reactome: R-HSA-168928
Reactome: R-HSA-5689880
Reactome: R-HSA-5689896
Reactome: R-HSA-918233
Reactome: R-HSA-933541
Reactome: R-HSA-933542
Reactome: R-HSA-933543
Reactome: R-HSA-936440
Reactome: R-HSA-9692916
Reactome: R-HSA-9705671
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Upon Viral Rna Stimulation And Isgylation
Translocates From Cytosol To Mitochondrion
May Be Found In The Nucleus
During Apoptosis
Alternative ELISA Names Interferon-Induced Helicase C Domain-Containing Protein 1 ELISA kit
Clinically Amyopathic Dermatomyositis Autoantigen 140 Kda ELISA kit
Cadm-140 Autoantigen ELISA kit
Helicase With 2 Card Domains ELISA kit
Helicard ELISA kit
Interferon-Induced With Helicase C Domain Protein 1 ELISA kit
Melanoma Differentiation-Associated Protein 5 ELISA kit
Mda-5 ELISA kit
Murabutide Down-Regulated Protein ELISA kit
Rig-I-Like Receptor 2 ELISA kit
Rlr-2 ELISA kit
Rna Helicase-Dead Box Protein 116 ELISA kit
IFIH1 ELISA kit
MDA5 ELISA kit
RH116 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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