产品详情
查看大图

  • 产品名称：Anti-CARM1 antibody (STJA0010244)

  • 产品型号：STJA0010244
  • 产品厂商：ST john's laboratory
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Anti-CARM1 antibody (STJA0010244)
详情介绍：
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histone-arginine methyltransferase CARM1 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CARM1
Gene ID: 10498
Uniprot ID: CARM1_HUMAN
Specificity: This antibody detects endogenous levels of Human, Mouse CARM1
Immunogen: Synthesized peptide derived from human CARM1
Tissue Specificity Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
Post Translational Modifications Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing. Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol. Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity. Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO9 at Lys-227.leading to proteasomal degradation.
Function Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. Acts as a transcriptional coactivator of ACACA/acetyl-CoA carboxylase by enriching H3R17 methylation at its promoter, thereby positively regulating fatty acid synthesis. Independently of its methyltransferase activity, involved in replication fork progression: promotes PARP1 recruitment to replication forks, leading to poly-ADP-ribosylation of chromatin at replication forks and reduced fork speed.
Protein Name Histone-Arginine Methyltransferase Carm1
Coactivator-Associated Arginine Methyltransferase 1
Protein Arginine N-Methyltransferase 4
Database Links Reactome: R-HSA-1368082
Reactome: R-HSA-1368108
Reactome: R-HSA-1989781
Reactome: R-HSA-2151201
Reactome: R-HSA-2426168
Reactome: R-HSA-3214858
Reactome: R-HSA-381340
Reactome: R-HSA-400206
Reactome: R-HSA-400253
Reactome: R-HSA-6804114
Reactome: R-HSA-9018519
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Cellular Localisation Nucleus
Cytoplasm
Chromosome
Mainly Nuclear During The G1
S And G2 Phases Of The Cell Cycle
Cytoplasmic During Mitosis
After Breakup Of The Nuclear Membrane
Localizes To Replication Forks
Alternative Antibody Names Anti-Histone-Arginine Methyltransferase Carm1 antibody
Anti-Coactivator-Associated Arginine Methyltransferase 1 antibody
Anti-Protein Arginine N-Methyltransferase 4 antibody
Anti-CARM1 antibody
Anti-PRMT4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
姓名：
电话：
您的需求：
 

沪公网安备 31011002002624号