产品详情
简单介绍：
Human IGF2BP3 (Insulin-like growth factor 2 mRNA-binding protein 3) CLIA Kit (STJC0000255)
详情介绍：
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 37.5pg/mL |
| Detection Limit: | 62.5~4000pg/mL |
| Short Description: | This human IGF2BP3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | IGF2BP3 |
| Gene ID: | 10643 |
| Uniprot ID: | IF2B3_HUMAN |
| Specificity: | This kit recognizes Human IGF2BP3 in samples. No significant cross-reactivity or interference between Human IGF2BP3 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines. |
| Function | RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. |
| Protein Name | Insulin-Like Growth Factor 2 Mrna-Binding Protein 3 Igf2 Mrna-Binding Protein 3 Imp-3 Igf-Ii Mrna-Binding Protein 3 Kh Domain-Containing Protein Overexpressed In Cancer Hkoc Vickz Family Member 3 |
| Database Links | Reactome: R-HSA-428359 |
| Cellular Localisation | Nucleus Cytoplasm P-Body Stress Granule Found In Lamellipodia Of The Leading Edge In The Perinuclear Region And Beneath The Plasma Membrane The Subcytoplasmic Localization Is Cell Specific And Regulated By Cell Contact And Growth Localized At The Connecting Piece And The Tail Of The Spermatozoa Colocalized With Cd44 Mrna In Rnp Granules In Response To Cellular Stress Such As Oxidative Stress Recruited To Stress Granules |
| Alternative CLIA Names | Insulin-Like Growth Factor 2 Mrna-Binding Protein 3 CLIA kit Igf2 Mrna-Binding Protein 3 CLIA kit Imp-3 CLIA kit Igf-Ii Mrna-Binding Protein 3 CLIA kit Kh Domain-Containing Protein Overexpressed In Cancer CLIA kit Hkoc CLIA kit Vickz Family Member 3 CLIA kit IGF2BP3 CLIA kit IMP3 CLIA kit KOC1 CLIA kit VICKZ3 CLIA kit |
| Specificity | This kit recognizes Human IGF2BP3 in samples. No significant cross-reactivity or interference between Human IGF2BP3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 98-113 | 105 |
| EDTA plasma(n=8) | 94-111 | 102 |
| Cell culture media(n=8) | 97-112 | 103 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 182.69 | 552.79 | 1734.35 | 191.17 | 540.37 | 1735.17 |
| Standard deviation | 17.39 | 42.01 | 187.31 | 20.95 | 64.79 | 135.69 |
| CV (%) | 9.52 | 7.60 | 10.80 | 10.96 | 11.99 | 7.82 |
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