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  • 产品名称：Human IL11 (Interleukin-11) CLIA Kit (STJC0001002)

  • 产品型号：STJC0001002
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL11 (Interleukin-11) CLIA Kit (STJC0001002)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human IL11 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IL11
Gene ID: 3589
Uniprot ID: IL11_HUMAN
Specificity: This kit recognizes Human IL-11 in samples. No significant cross-reactivity or interference between Human IL-11 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling'.
Protein Name Interleukin-11
Il-11
Adipogenesis Inhibitory Factor
Agif
Oprelvekin
Database Links Reactome: R-HSA-6788467
Cellular Localisation Secreted
Alternative CLIA Names Interleukin-11 CLIA kit
Il-11 CLIA kit
Adipogenesis Inhibitory Factor CLIA kit
Agif CLIA kit
Oprelvekin CLIA kit
IL11 CLIA kit
Specificity This kit recognizes Human IL-11 in samples. No significant cross-reactivity or interference between Human IL-11 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-113 107
EDTA plasma(n=8) 101-113 107
Cell culture media(n=8) 93-111 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 40.73 116.68 312.97 44.21 109.03 284.76
Standard deviation 3.91 9.57 30.80 5.13 8.96 17.60
CV (%) 9.60 8.20 9.84 11.60 8.22 6.18
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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