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  • 产品名称：Human IL15 (Interleukin-15) Sandwich ELISA (STJE0002063)

  • 产品型号：STJE0002063
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL15 (Interleukin-15) Sandwich ELISA (STJE0002063)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This IL15 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL15
Gene ID: 3600
Uniprot ID: IL15_HUMAN
Specificity: This kit recognizes Human IL-15 in samples. No significant cross-reactivity or interference between Human IL-15 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems. Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines. In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection. Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits. Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5. In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4.
Protein Name Interleukin-15
Il-15
Database Links Reactome: R-HSA-8983432
Cellular Localisation Isoform Il15-S48aa: Secreted
Isoform Il15-S21aa: Cytoplasm
Nucleus
Il15-S21aa Is Not Secreted
But Rather Is Stored Intracellularly
Appearing In The Nucleus And Cytoplasmic Components
Alternative ELISA Names Interleukin-15 ELISA kit
Il-15 ELISA kit
IL15 ELISA kit
Specificity This kit recognizes Human IL-15 in samples. No significant cross-reactivity or interference between Human IL-15 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-106 98
EDTA plasma (n=8) 92-103 97
Cell culture media (n=8) 88-103 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.35 146.58 348.04 46.47 145.32 338.28
Standard deviation 3.02 8.27 15.04 3.16 6.47 18.37
CV (%) 6.38 5.64 4.32 6.80 4.45 5.43
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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