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  • 产品名称：Human IL1R1 (Interleukin-1 receptor type 1) Sandwich ELISA (STJE0002666)

  • 产品型号：STJE0002666
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL1R1 (Interleukin-1 receptor type 1) Sandwich ELISA (STJE0002666)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This IL1R1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL1R1
Gene ID: 3554
Uniprot ID: IL1R1_HUMAN
Specificity: This kit recognizes Human IL-1R1 in samples. No significant cross-reactivity or interference between Human IL-1R1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications A soluble form (sIL1R1) is probably produced by proteolytic cleavage at the cell surface (shedding). Rapidly phosphorylated on Tyr-496 in response to IL-1, which creates a SH2 binding site for the PI 3-kinase regulatory subunit PIK3R1.
Function Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.
Protein Name Interleukin-1 Receptor Type 1
Il-1r-1
Il-1rt-1
Il-1rt1
Cd121 Antigen-Like Family Member A
Interleukin-1 Receptor Alpha
Il-1r-Alpha
Interleukin-1 Receptor Type I
P80
Cd Antigen Cd121a Cleaved Into - Interleukin-1 Receptor Type 1 - Membrane Form
Mil-1r1
Mil-1ri - Interleukin-1 Receptor Type 1 - Soluble Form
Sil-1r1
Sil-1ri
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-9020702
Reactome: R-HSA-9679191
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cell Membrane
Secreted
Alternative ELISA Names Interleukin-1 Receptor Type 1 ELISA kit
Il-1r-1 ELISA kit
Il-1rt-1 ELISA kit
Il-1rt1 ELISA kit
Cd121 Antigen-Like Family Member A ELISA kit
Interleukin-1 Receptor Alpha ELISA kit
Il-1r-Alpha ELISA kit
Interleukin-1 Receptor Type I ELISA kit
P80 ELISA kit
Cd Antigen Cd121a Cleaved Into - Interleukin-1 Receptor Type 1 - Membrane Form ELISA kit
Mil-1r1 ELISA kit
Mil-1ri - Interleukin-1 Receptor Type 1 - Soluble Form ELISA kit
Sil-1r1 ELISA kit
Sil-1ri ELISA kit
IL1R1 ELISA kit
IL1R ELISA kit
IL1RA ELISA kit
IL1RT1 ELISA kit
Specificity This kit recognizes Human IL-1R1 in samples. No significant cross-reactivity or interference between Human IL-1R1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 92
EDTA plasma (n=8) 92-104 97
Cell culture media (n=8) 92-107 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 1.13 4.95 0.47 1.17 5.39
Standard deviation 0.03 0.06 0.21 0.03 0.06 0.27
CV (%) 6.00 5.31 4.24 6.38 5.13 5.01
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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