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  • 产品名称：Human IL1RN (Interleukin-1 receptor antagonist protein) CLIA Kit (STJC0000082)

  • 产品型号：STJC0000082
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL1RN (Interleukin-1 receptor antagonist protein) CLIA Kit (STJC0000082)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human IL1RN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IL1RN
Gene ID: 3557
Uniprot ID: IL1RA_HUMAN
Specificity: This kit recognizes Human IL-1ra in samples. No significant cross-reactivity or interference between Human IL-1ra and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Function Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
Protein Name Interleukin-1 Receptor Antagonist Protein
Il-1rn
Il-1ra
Irap
Icil-1ra
Il1 Inhibitor
Anakinra
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-9020702
Cellular Localisation Isoform 1: Secreted
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Isoform 4: Cytoplasm
Alternative CLIA Names Interleukin-1 Receptor Antagonist Protein CLIA kit
Il-1rn CLIA kit
Il-1ra CLIA kit
Irap CLIA kit
Icil-1ra CLIA kit
Il1 Inhibitor CLIA kit
Anakinra CLIA kit
IL1RN CLIA kit
IL1F3 CLIA kit
IL1RA CLIA kit
Specificity This kit recognizes Human IL-1ra in samples. No significant cross-reactivity or interference between Human IL-1ra and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 93
EDTA plasma(n=8) 100-116 108
Cell culture media(n=8) 90-104 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.24 143.89 386.21 48.10 134.58 388.18
Standard deviation 5.99 10.86 34.95 5.34 10.58 39.83
CV (%) 12.68 7.55 9.05 11.10 7.86 10.26
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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