产品详情
简单介绍：
Human IL1RN (Interleukin-1 receptor antagonist protein) Sandwich ELISA (STJE0001980)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This IL1RN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | IL1RN |
| Gene ID: | 3557 |
| Uniprot ID: | IL1RA_HUMAN |
| Specificity: | This kit recognizes Human IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Human IL-1ra/IL-1F3 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens. |
| Protein Name | Interleukin-1 Receptor Antagonist Protein Il-1rn Il-1ra Irap Icil-1ra Il1 Inhibitor Anakinra |
| Database Links | Reactome: R-HSA-6783783 Reactome: R-HSA-9020702 |
| Cellular Localisation | Isoform 1: Secreted Isoform 2: Cytoplasm Isoform 3: Cytoplasm Isoform 4: Cytoplasm |
| Alternative ELISA Names | Interleukin-1 Receptor Antagonist Protein ELISA kit Il-1rn ELISA kit Il-1ra ELISA kit Irap ELISA kit Icil-1ra ELISA kit Il1 Inhibitor ELISA kit Anakinra ELISA kit IL1RN ELISA kit IL1F3 ELISA kit IL1RA ELISA kit |
| Specificity | This kit recognizes Human IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Human IL-1ra/IL-1F3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 95-110 | 101 |
| EDTA plasma (n=8) | 92-108 | 99 |
| Cell culture media (n=8) | 88-101 | 95 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 96.60 | 253.23 | 775.71 | 104.45 | 248.93 | 801.14 |
| Standard deviation | 5.12 | 13.12 | 41.11 | 5.89 | 12.92 | 43.58 |
| CV (%) | 5.30 | 5.18 | 5.30 | 5.64 | 5.19 | 5.44 |
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