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  • 产品名称：Human GRIN1 (Glutamate receptor ionotropic, NMDA 1) Sandwich ELISA (STJE0003117)

  • 产品型号：STJE0003117
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human GRIN1 (Glutamate receptor ionotropic, NMDA 1) Sandwich ELISA (STJE0003117)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This GRIN1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GRIN1
Gene ID: 2902
Uniprot ID: NMDZ1_HUMAN
Specificity: This kit recognizes Human GRIN1 in samples. No significant cross-reactivity or interference between Human GRIN1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.
Protein Name Glutamate Receptor Ionotropic - Nmda 1
Glun1
Glutamate Nmda Receptor Subunit Zeta-1
N-Methyl-D-Aspartate Receptor Subunit Nr1
Nmd-R1
Database Links Reactome: R-HSA-3928662
Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-6794361
Reactome: R-HSA-8849932
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Postsynaptic Density
Enriched In Postsynaptic Plasma Membrane And Postsynaptic Densities
Alternative ELISA Names Glutamate Receptor Ionotropic - Nmda 1 ELISA kit
Glun1 ELISA kit
Glutamate Nmda Receptor Subunit Zeta-1 ELISA kit
N-Methyl-D-Aspartate Receptor Subunit Nr1 ELISA kit
Nmd-R1 ELISA kit
GRIN1 ELISA kit
NMDAR1 ELISA kit
Specificity This kit recognizes Human GRIN1 in samples. No significant cross-reactivity or interference between Human GRIN1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 95
EDTA plasma (n=8) 90-102 96
Cell culture media (n=8) 94-106 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.47 1.57 4.77 0.43 1.51 4.40
Standard deviation 0.02 0.07 0.25 0.03 0.06 0.21
CV (%) 4.26 4.46 5.24 6.98 3.97 4.77
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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