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  • 产品名称：Human IgG (Immunoglobulin G) Sandwich ELISA (STJE0002030)

  • 产品型号：STJE0002030
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IgG (Immunoglobulin G) Sandwich ELISA (STJE0002030)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.94ng/mL
Detection Limit: 1.56~100ng/mL
Short Description: This IgG Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IGHG3Uniprot ID=IGHG3_HUMAN"
Specificity: This kit recognizes Human IgG in samples. No significant cross-reactivity or interference between Human IgG and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
Protein Name Immunoglobulin Heavy Constant Gamma 3
Hdc
Heavy Chain Disease Protein
Ig Gamma-3 Chain C Region
Cellular Localisation Isoform 1: Secreted
Isoform 2: Cell Membrane
Single-Pass Membrane Protein
Alternative ELISA Names Immunoglobulin Heavy Constant Gamma 3 ELISA kit
Hdc ELISA kit
Heavy Chain Disease Protein ELISA kit
Ig Gamma-3 Chain C Region ELISA kit
IGHG3 ELISA kit
Specificity This kit recognizes Human IgG in samples. No significant cross-reactivity or interference between Human IgG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-102 93
EDTA plasma (n=8) 91-107 98
Cell culture media (n=8) 87-103 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 4.70 12.50 33.60 5.10 11.50 36.50
Standard deviation 0.30 0.60 1.50 0.30 0.50 1.60
CV (%) 6.38 4.80 4.46 5.88 4.35 4.38
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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