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  • 产品名称：Human IL3 (Interleukin-3) CLIA Kit (STJC0000090)

  • 产品型号：STJC0000090
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL3 (Interleukin-3) CLIA Kit (STJC0000090)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This human IL3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IL3
Gene ID: 3562
Uniprot ID: IL3_HUMAN
Specificity: This kit recognizes Human IL-3 in samples. No significant cross-reactivity or interference between Human IL-3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Activated T-cells, mast cells, natural killer cells.
Function Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK.
Protein Name Interleukin-3
Il-3
Hematopoietic Growth Factor
Mast Cell Growth Factor
Mcgf
Multipotential Colony-Stimulating Factor
P-Cell-Stimulating Factor
Database Links Reactome: R-HSA-512988
Reactome: R-HSA-5673001
Reactome: R-HSA-8939247
Reactome: R-HSA-912526
Cellular Localisation Secreted
Alternative CLIA Names Interleukin-3 CLIA kit
Il-3 CLIA kit
Hematopoietic Growth Factor CLIA kit
Mast Cell Growth Factor CLIA kit
Mcgf CLIA kit
Multipotential Colony-Stimulating Factor CLIA kit
P-Cell-Stimulating Factor CLIA kit
IL3 CLIA kit
Specificity This kit recognizes Human IL-3 in samples. No significant cross-reactivity or interference between Human IL-3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 101-113 106
EDTA plasma(n=8) 88-99 93
Cell culture media(n=8) 87-99 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 22.79 79.90 222.34 24.00 72.97 209.45
Standard deviation 2.29 6.26 14.45 2.69 8.36 16.38
CV (%) 10.05 7.83 6.50 11.21 11.46 7.82
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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