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  • 产品名称：Human IL33 (Interleukin-33) CLIA Kit (STJC0000974)

  • 产品型号：STJC0000974
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL33 (Interleukin-33) CLIA Kit (STJC0000974)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This human IL33 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IL33
Gene ID: 90865
Uniprot ID: IL33_HUMAN
Specificity: This kit recognizes Human IL-33 in samples. No significant cross-reactivity or interference between Human IL-33 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
Post Translational Modifications The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains. Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation. In vitro proteolytic cleavage by CASP1 was reported but could not be confirmed in vivo suggesting that IL33 is probably not a direct substrate for that caspase.
Function Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as an enhancer of polarization of alternatively activated macrophages. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury. Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or pro-inflammatory activation.
Protein Name Interleukin-33
Il-33
Interleukin-1 Family Member 11
Il-1f11
Nuclear Factor From High Endothelial Venules
Nf-Hev Cleaved Into - Interleukin-33
95-270 - Interleukin-33
99-270 - Interleukin-33
109-270
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-5689880
Reactome: R-HSA-6811558
Reactome: R-HSA-9014843
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Cytoplasmic Vesicle
Secretory Vesicle
Secreted
Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore Following Cleavage By Cela1
Associates With Heterochromatin And Mitotic Chromosomes
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative CLIA Names Interleukin-33 CLIA kit
Il-33 CLIA kit
Interleukin-1 Family Member 11 CLIA kit
Il-1f11 CLIA kit
Nuclear Factor From High Endothelial Venules CLIA kit
Nf-Hev Cleaved Into - Interleukin-33 CLIA kit
95-270 - Interleukin-33 CLIA kit
99-270 - Interleukin-33 CLIA kit
109-270 CLIA kit
IL33 CLIA kit
C9orf26 CLIA kit
IL1F11 CLIA kit
NFHEV CLIA kit
Specificity This kit recognizes Human IL-33 in samples. No significant cross-reactivity or interference between Human IL-33 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-102 97
EDTA plasma(n=8) 95-108 101
Cell culture media(n=8) 92-105 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 24.16 68.85 187.61 26.17 64.40 182.87
Standard deviation 2.98 5.00 14.01 3.13 5.57 19.80
CV (%) 12.33 7.26 7.47 11.96 8.65 10.83
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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