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  • 产品名称：Human IL4 (Interleukin-4) CLIA Kit (STJC0000091)

  • 产品型号：STJC0000091
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IL4 (Interleukin-4) CLIA Kit (STJC0000091)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human IL4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IL4
Gene ID: 3565
Uniprot ID: IL4_HUMAN
Specificity: This kit recognizes Human IL-4 in samples. No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells. Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6.
Protein Name Interleukin-4
Il-4
B-Cell Stimulatory Factor 1
Bsf-1
Binetrakin
Lymphocyte Stimulatory Factor 1
Pitrakinra
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-9012546
Cellular Localisation Secreted
Alternative CLIA Names Interleukin-4 CLIA kit
Il-4 CLIA kit
B-Cell Stimulatory Factor 1 CLIA kit
Bsf-1 CLIA kit
Binetrakin CLIA kit
Lymphocyte Stimulatory Factor 1 CLIA kit
Pitrakinra CLIA kit
IL4 CLIA kit
Specificity This kit recognizes Human IL-4 in samples. No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 99
EDTA plasma(n=8) 87-102 95
Cell culture media(n=8) 93-104 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 41.60 103.91 309.08 43.94 109.99 299.01
Standard deviation 5.15 9.58 28.59 4.53 10.74 23.89
CV (%) 12.38 9.22 9.25 10.31 9.76 7.99
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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