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  • 产品名称：Human IRF5 (Interferon regulatory factor 5) CLIA Kit (STJC0000691)

  • 产品型号：STJC0000691
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human IRF5 (Interferon regulatory factor 5) CLIA Kit (STJC0000691)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human IRF5 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IRF5
Gene ID: 3663
Uniprot ID: IRF5_HUMAN
Specificity: This kit recognizes Human IRF5 in samples. No significant cross-reactivity or interference between Human IRF5 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylation of serine and threonine residues by IKBKB in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/EP300 and initiation of transcription. 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.
Function Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway. Key transcription factor regulating the IFN response during SARS-CoV-2 infection.
Protein Name Interferon Regulatory Factor 5
Irf-5
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-909733
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between The Nucleus And The Cytoplasm: Upon Activation By The Tlr Adapter Myd88 And Subsequent Phosphorylation
Translocates To The Nucleus
Alternative CLIA Names Interferon Regulatory Factor 5 CLIA kit
Irf-5 CLIA kit
IRF5 CLIA kit
Specificity This kit recognizes Human IRF5 in samples. No significant cross-reactivity or interference between Human IRF5 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-105 98
EDTA plasma(n=8) 94-105 100
Cell culture media(n=8) 92-106 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 94.57 177.11 685.58 99.81 177.06 668.88
Standard deviation 10.12 20.99 43.33 12.89 18.63 40.13
CV (%) 10.70 11.85 6.32 12.91 10.52 6.00
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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