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  • 产品名称：Human ITLN1 (Intelectin-1) CLIA Kit (STJC0000862)

  • 产品型号：STJC0000862
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human ITLN1 (Intelectin-1) CLIA Kit (STJC0000862)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 75pg/mL
Detection Limit: 125~8000pg/mL
Short Description: This human ITLN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: ITLN1
Gene ID: 55600
Uniprot ID: ITLN1_HUMAN
Specificity: This kit recognizes Human ITLN1 in samples. No significant cross-reactivity or interference between Human ITLN1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Highly expressed in omental adipose tissue where it is found in stromal vascular cells but not in fat cells but is barely detectable in subcutaneous adipose tissue (at protein level). Highly expressed in the small intestine. Also found in the heart, testis, colon, salivary gland, skeletal muscle, pancreas and thyroid and, to a lesser degree, in the uterus, spleen, prostate, lymph node and thymus.
Post Translational Modifications N-glycosylated.
Function Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner. Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris. Does not bind human glycans. Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption.
Protein Name Intelectin-1
Itln-1
Endothelial Lectin Hl-1
Galactofuranose-Binding Lectin
Intestinal Lactoferrin Receptor
Omentin
Database Links Reactome: R-HSA-6803157
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Secreted
Enriched In Lipid Rafts
Alternative CLIA Names Intelectin-1 CLIA kit
Itln-1 CLIA kit
Endothelial Lectin Hl-1 CLIA kit
Galactofuranose-Binding Lectin CLIA kit
Intestinal Lactoferrin Receptor CLIA kit
Omentin CLIA kit
ITLN1 CLIA kit
INTL CLIA kit
ITLN CLIA kit
LFR CLIA kit
UNQ640 CLIA kit
PRO1270 CLIA kit
Specificity This kit recognizes Human ITLN1 in samples. No significant cross-reactivity or interference between Human ITLN1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-114 107
EDTA plasma(n=8) 85-95 90
Cell culture media(n=8) 86-101 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 368.73 841.43 3720.56 335.76 867.97 3433.36
Standard deviation 41.85 100.30 231.05 32.43 72.48 270.55
CV (%) 11.35 11.92 6.21 9.66 8.35 7.88
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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