产品详情
简单介绍：
Human JAK1 (Tyrosine-protein kinase JAK1) Sandwich ELISA (STJE0003925)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description: | This JAK1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | JAK1 |
| Gene ID: | 3716 |
| Uniprot ID: | JAK1_HUMAN |
| Specificity: | This kit recognizes Human JAK1 in samples. No significant cross-reactivity or interference between Human JAK1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Autophosphorylated. Phosphorylated by TYK2 on tyrosine residues in response to type-I interferon signaling, leading to its activation. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling. Ubiquitinated by RNF125.leading to its degradation by the proteasome. |
| Function | Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor. Kinase partner for the type I interferon receptor IFNAR2. In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins. Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors. |
| Protein Name | Tyrosine-Protein Kinase Jak1 Janus Kinase 1 Jak-1 |
| Database Links | Reactome: R-HSA-1059683 Reactome: R-HSA-110056 Reactome: R-HSA-112411 Reactome: R-HSA-1169408 Reactome: R-HSA-1266695 Reactome: R-HSA-449836 Reactome: R-HSA-5673001 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-6788467 Reactome: R-HSA-877300 Reactome: R-HSA-877312 Reactome: R-HSA-8854691 Reactome: R-HSA-8983432 Reactome: R-HSA-8984722 Reactome: R-HSA-8985947 Reactome: R-HSA-9020558 Reactome: R-HSA-9020591 Reactome: R-HSA-9020956 Reactome: R-HSA-9020958 Reactome: R-HSA-909733 Reactome: R-HSA-912526 Reactome: R-HSA-912694 Reactome: R-HSA-9674555 Reactome: R-HSA-9679191 Reactome: R-HSA-9705462 Reactome: R-HSA-9705671 Reactome: R-HSA-9732724 |
| Cellular Localisation | Endomembrane System Peripheral Membrane Protein Wholly Intracellular Possibly Membrane Associated |
| Alternative ELISA Names | Tyrosine-Protein Kinase Jak1 ELISA kit Janus Kinase 1 ELISA kit Jak-1 ELISA kit JAK1 ELISA kit JAK1A ELISA kit JAK1B ELISA kit |
| Specificity | This kit recognizes Human JAK1 in samples. No significant cross-reactivity or interference between Human JAK1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 91-105 | 97 |
| EDTA plasma (n=8) | 88-102 | 95 |
| Cell culture media (n=8) | 86-100 | 92 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.49 | 1.02 | 3.44 | 0.53 | 0.93 | 3.44 |
| Standard deviation | 0.03 | 0.05 | 0.16 | 0.04 | 0.05 | 0.17 |
| CV (%) | 6.12 | 4.90 | 4.65 | 7.55 | 5.38 | 4.94 |
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