产品详情
简单介绍:
Human LDHA (L-lactate dehydrogenase A chain) CLIA Kit (STJC0000310)
详情介绍:
Applications: | CLIA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 0.09ng/mL |
Detection Limit: | 0.16~10ng/mL |
Short Description: | This human LDHA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Chemiluminescence |
Gene Symbol: | LDHA |
Gene ID: | 3939 |
Uniprot ID: | LDHA_HUMAN |
Specificity: | This kit recognizes Human LDHA in samples. No significant cross-reactivity or interference between Human LDHA and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Tissue Specificity | Predominantly expressed in anaerobic tissues such as skeletal muscle and liver. |
Post Translational Modifications | ISGylated. |
Function | Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+). |
Protein Name | L-Lactate Dehydrogenase A Chain Ldh-A Cell Proliferation-Inducing Gene 19 Protein Ldh Muscle Subunit Ldh-M Renal Carcinoma Antigen Ny-Ren-59 |
Database Links | Reactome: R-HSA-70268 |
Cellular Localisation | Cytoplasm |
Alternative CLIA Names | L-Lactate Dehydrogenase A Chain CLIA kit Ldh-A CLIA kit Cell Proliferation-Inducing Gene 19 Protein CLIA kit Ldh Muscle Subunit CLIA kit Ldh-M CLIA kit Renal Carcinoma Antigen Ny-Ren-59 CLIA kit LDHA CLIA kit PIG19 CLIA kit |
Specificity | This kit recognizes Human LDHA in samples. No significant cross-reactivity or interference between Human LDHA and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item Specifications Storage
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type Range (%) Average Recovery (%)
Serum(n=8) | 91-102 | 96 |
EDTA plasma(n=8) | 97-111 | 105 |
Cell culture media(n=8) | 92-106 | 99 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 0.46 | 1.30 | 4.76 | 0.46 | 1.24 | 5.18 |
Standard deviation | 0.05 | 0.09 | 0.50 | 0.06 | 0.10 | 0.54 |
CV (%) | 10.87 | 6.92 | 10.50 | 13.04 | 8.06 | 10.42 |
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