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  • 产品名称：Human LGALS1 (Galectin-1) Sandwich ELISA (STJE0002682)

  • 产品型号：STJE0002682
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human LGALS1 (Galectin-1) Sandwich ELISA (STJE0002682)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This LGALS1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: LGALS1
Gene ID: 3956
Uniprot ID: LEG1_HUMAN
Specificity: This kit recognizes Human GAL1 in samples. No significant cross-reactivity or interference between Human GAL1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Protein Name Galectin-1
Gal-1
14 Kda Laminin-Binding Protein
Hlbp14
14 Kda Lectin
Beta-Galactoside-Binding Lectin L-14-I
Galaptin
Hbl
Hpl
Lactose-Binding Lectin 1
Lectin Galactoside-Binding Soluble 1
Putative Mapk-Activating Protein Pm12
S-Lac Lectin 1
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Cytoplasm
Can Be Secreted
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative ELISA Names Galectin-1 ELISA kit
Gal-1 ELISA kit
14 Kda Laminin-Binding Protein ELISA kit
Hlbp14 ELISA kit
14 Kda Lectin ELISA kit
Beta-Galactoside-Binding Lectin L-14-I ELISA kit
Galaptin ELISA kit
Hbl ELISA kit
Hpl ELISA kit
Lactose-Binding Lectin 1 ELISA kit
Lectin Galactoside-Binding Soluble 1 ELISA kit
Putative Mapk-Activating Protein Pm12 ELISA kit
S-Lac Lectin 1 ELISA kit
LGALS1 ELISA kit
Specificity This kit recognizes Human GAL1 in samples. No significant cross-reactivity or interference between Human GAL1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-105 100
EDTA plasma (n=8) 91-104 98
Cell culture media (n=8) 94-107 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.40 5.79 22.26 2.27 5.27 20.42
Standard deviation 0.14 0.28 1.19 0.14 0.29 0.82
CV (%) 5.83 4.84 5.35 6.17 5.50 4.02
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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