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  • 产品名称：Human LGALS9 (Galectin-9) Sandwich ELISA (STJE0002690)

  • 产品型号：STJE0002690
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human LGALS9 (Galectin-9) Sandwich ELISA (STJE0002690)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This LGALS9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: LGALS9
Gene ID: 3965
Uniprot ID: LEG9_HUMAN
Specificity: This kit recognizes Human GAL9 in samples. No significant cross-reactivity or interference between Human GAL9 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Binds galactosides. Has high affinity for the Forssman pentasaccharide. Ligand for HAVCR2/TIM3. Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death. Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth. Ligand for P4HB.the interaction retains P4HB at the cell surface of Th2 T-helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration. Ligand for CD44.the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function. Promotes ability of mesenchymal stromal cells to suppress T-cell proliferation. Expands regulatory T-cells and induces cytotoxic T-cell apoptosis following virus infection. Activates ERK1/2 phosphorylation inducing cytokine (IL-6, IL-8, IL-12) and chemokine (CCL2) production in mast and dendritic cells. Inhibits degranulation and induces apoptosis of mast cells. Induces maturation and migration of dendritic cells. Inhibits natural killer (NK) cell function. Can transform NK cell phenotype from peripheral to decidual during pregnancy. Astrocyte derived galectin-9 enhances microglial TNF production. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion. Highly selective to the anion urate. Isoform 2: Acts as an eosinophil chemoattractant. It also inhibits angiogenesis. Suppresses IFNG production by natural killer cells.
Protein Name Galectin-9
Gal-9
Ecalectin
Tumor Antigen Hom-Hd-21
Database Links Reactome: R-HSA-451927
Cellular Localisation Cytoplasm
Nucleus
Secreted
May Also Be Secreted By A Non-Classical Secretory Pathway
Secreted By Mesenchymal Stromal Cells Upon Ifng Stimulation
Isoform 2: Secreted
Isoform 3: Secreted
Alternative ELISA Names Galectin-9 ELISA kit
Gal-9 ELISA kit
Ecalectin ELISA kit
Tumor Antigen Hom-Hd-21 ELISA kit
LGALS9 ELISA kit
Specificity This kit recognizes Human GAL9 in samples. No significant cross-reactivity or interference between Human GAL9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-108 99
EDTA plasma (n=8) 83-96 90
Cell culture media (n=8) 92-105 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 22.95 70.96 204.84 24.81 71.23 194.40
Standard deviation 1.32 3.73 6.21 1.60 3.40 8.16
CV (%) 5.75 5.26 3.03 6.45 4.77 4.20
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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