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  • 产品名称：Human LpPLA2 (Lipoprotein-associated Phospholipase A2) Sandwich ELISA (STJE0003591)

  • 产品型号：STJE0003591
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human LpPLA2 (Lipoprotein-associated Phospholipase A2) Sandwich ELISA (STJE0003591)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This LpPLA2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PLA2G7
Gene ID: 7941
Uniprot ID: PAFA_HUMAN
Specificity: This kit recognizes Human LpPLA2 in samples. No significant cross-reactivity or interference between Human LpPLA2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated. Macrophage-derived PLA2G7 carries sialylated complex-type N-glycans that hinder its binding to HDL particles.
Function Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage.
Protein Name Platelet-Activating Factor Acetylhydrolase
Paf Acetylhydrolase
1-Alkyl-2-Acetylglycerophosphocholine Esterase
2-Acetyl-1-Alkylglycerophosphocholine Esterase
Group-Viia Phospholipase A2
Gviia-Pla2
Ldl-Associated Phospholipase A2
Ldl-Pla(2
Paf 2-Acylhydrolase
Database Links Reactome: R-HSA-422085
Cellular Localisation Secreted
Extracellular Space
Associates With Both Ldl And Hdl Particles In Plasma
Mainly Associates With Pro-Inflammatory Electronegative Ldl Particles
Alternative ELISA Names Platelet-Activating Factor Acetylhydrolase ELISA kit
Paf Acetylhydrolase ELISA kit
1-Alkyl-2-Acetylglycerophosphocholine Esterase ELISA kit
2-Acetyl-1-Alkylglycerophosphocholine Esterase ELISA kit
Group-Viia Phospholipase A2 ELISA kit
Gviia-Pla2 ELISA kit
Ldl-Associated Phospholipase A2 ELISA kit
Ldl-Pla(2 ELISA kit
Paf 2-Acylhydrolase ELISA kit
PLA2G7 ELISA kit
PAFAH ELISA kit
Specificity This kit recognizes Human LpPLA2 in samples. No significant cross-reactivity or interference between Human LpPLA2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-101 95
EDTA plasma (n=8) 86-100 93
Cell culture media (n=8) 88-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.04 2.92 9.75 1.07 2.88 9.79
Standard deviation 0.06 0.16 0.50 0.06 0.12 0.44
CV (%) 5.77 5.48 5.13 5.61 4.17 4.49
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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