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  • 产品名称：Human MIP-1 Alpha (Macrophage Inflammatory Protein 1 Alpha) Sandwich ELISA (STJE0019880)

  • 产品型号：STJE0019880
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human MIP-1 Alpha (Macrophage Inflammatory Protein 1 Alpha) Sandwich ELISA (STJE0019880)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.62 pg/mL
Detection Limit: 3.13-200 pg/mL
Short Description: This MIP-1 Alpha Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CCL3
Gene ID: 6348
Uniprot ID: CCL3_HUMAN
Specificity: This kit recognizes Human MIP-1 Alpha in samples. No significant cross-reactivity or interference between Human MIP-1 Alpha and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Post Translational Modifications N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
Function Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Protein Name C-C Motif Chemokine 3
G0/G1 Switch Regulatory Protein 19-1
Macrophage Inflammatory Protein 1-Alpha
Mip-1-Alpha
Pat 464.1
Sis-Beta
Small-Inducible Cytokine A3
Tonsillar Lymphocyte Ld78 Alpha Protein Cleaved Into - Mip-1-Alpha(4-69
Ld78-Alpha(4-69
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-6783783
Cellular Localisation Secreted
Alternative ELISA Names C-C Motif Chemokine 3 ELISA kit
G0/G1 Switch Regulatory Protein 19-1 ELISA kit
Macrophage Inflammatory Protein 1-Alpha ELISA kit
Mip-1-Alpha ELISA kit
Pat 464.1 ELISA kit
Sis-Beta ELISA kit
Small-Inducible Cytokine A3 ELISA kit
Tonsillar Lymphocyte Ld78 Alpha Protein Cleaved Into - Mip-1-Alpha(4-69 ELISA kit
Ld78-Alpha(4-69 ELISA kit
CCL3 ELISA kit
G0S19-1 ELISA kit
MIP1A ELISA kit
SCYA3 ELISA kit
Specificity This kit recognizes Human MIP-1α in samples. No significant cross-reactivity or interference between Human MIP-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-104 95
EDTA plasma (n=8) 94-108 99
Cell culture media (n=8) 91-103 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 10.25 20.84 78.04 10.29 22.66 72.89
Standard deviation 0.53 1.03 2.81 0.65 1.21 3.00
CV (%) 5.18 4.94 3.60 6.36 5.36 4.11
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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