产品详情
简单介绍：
Human MYL9 (Myosin regulatory light polypeptide 9) CLIA Kit (STJC0000377)
详情介绍：
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This human MYL9 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | MYL9 |
| Gene ID: | 10398 |
| Uniprot ID: | MYL9_HUMAN |
| Specificity: | This kit recognizes Human MYL9 in samples. No significant cross-reactivity or interference between Human MYL9 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Smooth muscle tissues and in some, but not all, nonmuscle cells. |
| Post Translational Modifications | Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation. |
| Function | Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation. |
| Protein Name | Myosin Regulatory Light Polypeptide 9 20 Kda Myosin Light Chain Lc20 Mlc-2c Myosin Rlc Myosin Regulatory Light Chain 2 - Smooth Muscle Isoform Myosin Regulatory Light Chain 9 Myosin Regulatory Light Chain Mrlc1 |
| Database Links | Reactome: R-HSA-3928663 Reactome: R-HSA-416572 Reactome: R-HSA-445355 Reactome: R-HSA-5625740 Reactome: R-HSA-5625900 Reactome: R-HSA-5627117 Reactome: R-HSA-5627123 Reactome: R-HSA-8936459 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cell Cortex Colocalizes With F-Actin Myh9 And Piezo1 At The Actomyosin Cortex In Myoblasts |
| Alternative CLIA Names | Myosin Regulatory Light Polypeptide 9 CLIA kit 20 Kda Myosin Light Chain CLIA kit Lc20 CLIA kit Mlc-2c CLIA kit Myosin Rlc CLIA kit Myosin Regulatory Light Chain 2 - Smooth Muscle Isoform CLIA kit Myosin Regulatory Light Chain 9 CLIA kit Myosin Regulatory Light Chain Mrlc1 CLIA kit MYL9 CLIA kit MLC2 CLIA kit MRLC1 CLIA kit MYRL2 CLIA kit |
| Specificity | This kit recognizes Human MYL9 in samples. No significant cross-reactivity or interference between Human MYL9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 93-108 | 100 |
| EDTA plasma(n=8) | 89-102 | 94 |
| Cell culture media(n=8) | 97-112 | 104 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.79 | 84.09 | 372.03 | 48.62 | 89.88 | 400.18 |
| Standard deviation | 5.96 | 6.25 | 27.12 | 5.84 | 8.39 | 38.10 |
| CV (%) | 11.51 | 7.43 | 7.29 | 12.01 | 9.33 | 9.52 |
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