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  • 产品名称：Human NOS1 (Nitric oxide synthase, brain) Sandwich ELISA (STJE0002447)

  • 产品型号：STJE0002447
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human NOS1 (Nitric oxide synthase, brain) Sandwich ELISA (STJE0002447)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This NOS1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: NOS1
Gene ID: 4842
Uniprot ID: NOS1_HUMAN
Specificity: This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Ubiquitinated.mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Protein Name Nitric Oxide Synthase 1
Constitutive Nos
Nc-Nos
Nos Type I
Neuronal Nos
N-Nos
Nnos
Nitric Oxide Synthase - Brain
Bnos
Peptidyl-Cysteine S-Nitrosylase Nos1
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-392154
Reactome: R-HSA-5578775
Cellular Localisation Cell Membrane
Sarcolemma
Peripheral Membrane Protein
Cell Projection
Dendritic Spine
In Skeletal Muscle
It Is Localized Beneath The Sarcolemma Of Fast-Twitch Muscle Fiber By Associating With The Dystrophin Glycoprotein Complex
In Neurons
Enriched In Dendritic Spines
Alternative ELISA Names Nitric Oxide Synthase 1 ELISA kit
Constitutive Nos ELISA kit
Nc-Nos ELISA kit
Nos Type I ELISA kit
Neuronal Nos ELISA kit
N-Nos ELISA kit
Nnos ELISA kit
Nitric Oxide Synthase - Brain ELISA kit
Bnos ELISA kit
Peptidyl-Cysteine S-Nitrosylase Nos1 ELISA kit
NOS1 ELISA kit
Specificity This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 92
EDTA plasma (n=8) 91-108 98
Cell culture media (n=8) 91-106 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 1.40 3.50 0.50 1.30 3.40
Standard deviation 0.03 0.07 0.19 0.03 0.05 0.16
CV (%) 6.00 5.00 5.43 6.00 3.85 4.71
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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