Name: Human NOS3 (Nitric oxide synthase, endothelial) Sandwich ELISA (STJE0002459)
Brand: ST john's laboratory
SKU: STJE0002459

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产品名称：Human NOS3 (Nitric oxide synthase, endothelial) Sandwich ELISA (STJE0002459)
产品型号：STJE0002459
产品厂商：ST john's laboratory
产品文档：

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简单介绍：

Human NOS3 (Nitric oxide synthase, endothelial) Sandwich ELISA (STJE0002459)

详情介绍：

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	37.5pg/mL
Detection Limit:	62.5~4000pg/mL
Short Description:	This NOS3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	NOS3
Gene ID:	4846
Uniprot ID:	NOS3_HUMAN
Specificity:	This kit recognizes Human NOS3/eNOS in samples. No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity.
Function	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Protein Name	Nitric Oxide Synthase 3 Constitutive Nos Cnos Ec-Nos Nos Type Iii Nosiii Nitric Oxide Synthase - Endothelial Endothelial Nos Enos
Database Links	Reactome: R-HSA-1222556 Reactome: R-HSA-1474151 Reactome: R-HSA-203615 Reactome: R-HSA-203641 Reactome: R-HSA-203754 Reactome: R-HSA-392154 Reactome: R-HSA-5218920 Reactome: R-HSA-9009391
Cellular Localisation	Cell Membrane Membrane Caveola Cytoplasm Cytoskeleton Golgi Apparatus Specifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell Cycle Which Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity
Alternative ELISA Names	Nitric Oxide Synthase 3 ELISA kit Constitutive Nos ELISA kit Cnos ELISA kit Ec-Nos ELISA kit Nos Type Iii ELISA kit Nosiii ELISA kit Nitric Oxide Synthase - Endothelial ELISA kit Endothelial Nos ELISA kit Enos ELISA kit NOS3 ELISA kit
Specificity	This kit recognizes Human NOS3/eNOS in samples. No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	93-108	101
EDTA plasma (n=8)	94-106	99
Cell culture media (n=8)	92-104	98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	186.70	451.70	1380.60	204.80	422.50	1440.90
Standard deviation	12.90	20.30	69.00	12.70	18.60	46.10
CV (%)	6.91	4.49	5.00	6.20	4.40	3.20

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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产品名称：Human NOS3 (Nitric oxide synthase, endothelial) Sandwich ELISA (STJE0002459)