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  • 产品名称：Human OAS1 (2 NA, 5 Oligoadenylate Synthetase 1) Sandwich ELISA Kit (STJE0011150)

  • 产品型号：STJE0011150
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human OAS1 (2 NA, 5 Oligoadenylate Synthetase 1) Sandwich ELISA Kit (STJE0011150)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 1.8ng/mL
Detection Limit: 4.68-300ng/mL
Short Description: This OAS1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: OAS1
Gene ID: 4938
Uniprot ID: OAS1_HUMAN
Sample Type: serum, plasma, cell lysates or other biological fluids.
Tissue Specificity Expressed in lungs.
Post Translational Modifications Isoform p46: Prenylated at C-terminal. C-terminal prenylation is necessary to initiate a block to SARS-CoV-2 and is associated with protection from severe COVID-1. The prenylated form is targeted to perinuclear structures rich in viral dsRNA, whereas the non-prenylated form is diffusely localized and unable to initiate a detectable block to SARS-CoV-2 replication (Probable). C-terminal prenylation is also necessary to initiate a block to cardiovirus EMCV (Probable). Isoform p42: Not prenylated at C-terminal. The non-prenylated form is diffusely localized and unable to initiate a detectable block to SARS-CoV-2 replication.
Function Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L. Isoform p46: When prenylated at C-terminal, acts as a double-stranded RNA (dsRNA) sensor specifically targeted to membranous replicative organelles in SARS coronavirus-2/SARS-CoV-2 infected cells where it binds to dsRNA structures in the SARS-CoV-2 5'-UTR and initiates a potent block to SARS-CoV-2 replication. Recognizes short stretches of dsRNA and activates RNase L. The binding is remarkably specific, with two conserved stem loops in the SARS-CoV-2 5'- untranslated region (UTR) constituting the principal viral target. The same mechanism is necessary to initiate a block to cardiovirus EMCV. Isoform p42: Not prenylated at C-terminal, is diffusely localized and unable to initiate a detectable block to SARS-CoV-2 replication.
Protein Name 2'-5'-Oligoadenylate Synthase 1
(2-5'Oligo(A Synthase 1
2-5a Synthase 1
E18/E16
P46/P42 Oas
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-8983711
Reactome: R-HSA-909733
Cellular Localisation Cytoplasm
Mitochondrion
Nucleus
Microsome
Endoplasmic Reticulum
Secreted
Associated With Different Subcellular Fractions Such As Mitochondrial
Nuclear
And Rough/Smooth Microsomal Fractions
Isoform P46: (Microbial Infection) In Sars Coronavirus-2/Sars-Cov-2 Infected Cells
Prenylated Form Localizes To Membranous Perinuclear Structures Reminiscent Of The Endoplasmic Reticulum Rich In Viral Dsrna Which Are Sars-Cov-2 Replicative Organelles
Isoform P42: (Microbial Infection) In Sars Coronavirus-2/Sars-Cov-2 Infected Cells
Since Its Not Prenylated
Is Diffusely Localized And Unable To Initiate A Detectable Block To Sars-Cov-2 Replication
Alternative ELISA Names 2'-5'-Oligoadenylate Synthase 1 ELISA kit
(2-5'Oligo(A Synthase 1 ELISA kit
2-5a Synthase 1 ELISA kit
E18/E16 ELISA kit
P46/P42 Oas ELISA kit
OAS1 ELISA kit
OIAS ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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