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  • 产品名称：Human PADI4 (Protein-arginine deiminase type-4) Sandwich ELISA (STJE0002882)

  • 产品型号：STJE0002882
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human PADI4 (Protein-arginine deiminase type-4) Sandwich ELISA (STJE0002882)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.11ng/mL
Detection Limit: 0.19~12ng/mL
Short Description: This PADI4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PADI4
Gene ID: 23569
Uniprot ID: PADI4_HUMAN
Specificity: This kit recognizes Human PADI4 in samples. No significant cross-reactivity or interference between Human PADI4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme.
Function Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Required for the formation of neutrophil extracellular traps (NETs).NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
Protein Name Protein-Arginine Deiminase Type-4
Hl-60 Pad
Peptidylarginine Deiminase Iv
Protein-Arginine Deiminase Type Iv
Database Links Reactome: R-HSA-3247509
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic Granule
Cytoplasmic Granules Of Eosinophils And Neutrophils
Alternative ELISA Names Protein-Arginine Deiminase Type-4 ELISA kit
Hl-60 Pad ELISA kit
Peptidylarginine Deiminase Iv ELISA kit
Protein-Arginine Deiminase Type Iv ELISA kit
PADI4 ELISA kit
PAD4 ELISA kit
PADI5 ELISA kit
PDI5 ELISA kit
Specificity This kit recognizes Human PADI4 in samples. No significant cross-reactivity or interference between Human PADI4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-97 91
EDTA plasma (n=8) 92-108 99
Cell culture media (n=8) 84-96 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.58 1.76 5.70 0.56 1.81 6.08
Standard deviation 0.03 0.09 0.21 0.03 0.09 0.26
CV (%) 5.17 5.11 3.68 5.36 4.97 4.28
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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