产品详情
简单介绍：
Human PLA2R1 (Secretory phospholipase A2 receptor) Sandwich ELISA (STJE0002692)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description: | This PLA2R1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | PLA2R1 |
| Gene ID: | 22925 |
| Uniprot ID: | PLA2R_HUMAN |
| Specificity: | This kit recognizes Human PLA2R1 in samples. No significant cross-reactivity or interference between Human PLA2R1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form. |
| Function | Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in pro-inflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis. |
| Protein Name | Secretory Phospholipase A2 Receptor Pla2-R Pla2r 180 Kda Secretory Phospholipase A2 Receptor C-Type Lectin Domain Family 13 Member C M-Type Receptor Cleaved Into - Soluble Secretory Phospholipase A2 Receptor Soluble Pla2-R Soluble Pla2r |
| Database Links | Reactome: R-HSA-1482788 Reactome: R-HSA-1482801 Reactome: R-HSA-1482839 Reactome: R-HSA-1482922 Reactome: R-HSA-1482925 Reactome: R-HSA-1483166 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Soluble Secretory Phospholipase A2 Receptor: Secreted Isoform 2: Secreted |
| Alternative ELISA Names | Secretory Phospholipase A2 Receptor ELISA kit Pla2-R ELISA kit Pla2r ELISA kit 180 Kda Secretory Phospholipase A2 Receptor ELISA kit C-Type Lectin Domain Family 13 Member C ELISA kit M-Type Receptor Cleaved Into - Soluble Secretory Phospholipase A2 Receptor ELISA kit Soluble Pla2-R ELISA kit Soluble Pla2r ELISA kit PLA2R1 ELISA kit CLEC13C ELISA kit |
| Specificity | This kit recognizes Human PLA2R1 in samples. No significant cross-reactivity or interference between Human PLA2R1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item Specifications Storage
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
Sample Type Range (%) Average Recovery (%)
| Serum(n=8) | 87-98 | 93 |
| EDTA plasma (n=8) | 87-101 | 94 |
| Cell culture media (n=8) | 86-101 | 93 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.47 | 1.28 | 4.98 | 0.43 | 1.16 | 4.94 |
| Standard deviation | 0.02 | 0.06 | 0.22 | 0.03 | 0.06 | 0.19 |
| CV (%) | 4.26 | 4.69 | 4.42 | 6.98 | 5.17 | 3.85 |
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