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  • 产品名称：Human PPIA (Peptidyl-prolyl cis-trans isomerase A) Sandwich ELISA (STJE0004108)

  • 产品型号：STJE0004108
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human PPIA (Peptidyl-prolyl cis-trans isomerase A) Sandwich ELISA (STJE0004108)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.88ng/mL
Detection Limit: 3.13~200ng/mL
Short Description: This PPIA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PPIA
Gene ID: 5478
Uniprot ID: PPIA_HUMAN
Specificity: This kit recognizes Human CYPA in samples. No significant cross-reactivity or interference between Human CYPA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2. Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1. Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates. Plays an important role in platelet activation and aggregation. Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton. Binds heparan sulfate glycosaminoglycans. Inhibits replication of influenza A virus (IAV). Inhibits ITCH/AIP4-mediated ubiquitination of matrix protein 1 (M1) of IAV by impairing the interaction of ITCH/AIP4 with M1, followed by the suppression of the nuclear export of M1, and finally reduction of the replication of IAV. (Microbial infection) May act as a mediator between human SARS coronavirus nucleoprotein and BSG/CD147 in the process of invasion of host cells by the virus. (Microbial infection) Stimulates RNA-binding ability of HCV NS5A in a peptidyl-prolyl cis-trans isomerase activity-dependent manner.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase A
Ppiase A
Cyclophilin A
Cyclosporin A-Binding Protein
Rotamase A Cleaved Into - Peptidyl-Prolyl Cis-Trans Isomerase A - N-Terminally Processed
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-162585
Reactome: R-HSA-162588
Reactome: R-HSA-162592
Reactome: R-HSA-162594
Reactome: R-HSA-164516
Reactome: R-HSA-164525
Reactome: R-HSA-173107
Reactome: R-HSA-175474
Reactome: R-HSA-180689
Reactome: R-HSA-2025928
Reactome: R-HSA-210991
Reactome: R-HSA-6798695
Reactome: R-HSA-8950505
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Secreted
Nucleus
Secretion Occurs In Response To Oxidative Stress In Vascular Smooth Muscle Through A Vesicular Secretory Pathway That Includes Rho Gtpase Signaling
Actin Remodeling
And Myosin Ii Activation
Alternative ELISA Names Peptidyl-Prolyl Cis-Trans Isomerase A ELISA kit
Ppiase A ELISA kit
Cyclophilin A ELISA kit
Cyclosporin A-Binding Protein ELISA kit
Rotamase A Cleaved Into - Peptidyl-Prolyl Cis-Trans Isomerase A - N-Terminally Processed ELISA kit
PPIA ELISA kit
CYPA ELISA kit
Specificity This kit recognizes Human CYPA in samples. No significant cross-reactivity or interference between Human CYPA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-106 103
EDTA plasma (n=8) 95-103 98
Cell culture media (n=8) 92-100 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 8.29 20.91 90.96 9.58 22.80 97.65
Standard deviation 0.41 0.99 4.70 0.50 1.05 4.90
CV (%) 4.95 4.73 5.17 5.22 4.61 5.02
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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