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  • 产品名称：Human PRB1 (Basic salivary proline-rich protein 1) CLIA Kit (STJC0000520)

  • 产品型号：STJC0000520
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human PRB1 (Basic salivary proline-rich protein 1) CLIA Kit (STJC0000520)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human PRB1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: PRB1
Gene ID: 5542
Uniprot ID: PRP1_HUMAN
Specificity: This kit recognizes Human PRB1 in samples. No significant cross-reactivity or interference between Human PRB1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications O-glycosylated. O-glycosylation on Ser-87 is prevalent in head and neck cancer patients. O-Glycosylation on Ser-330 has a 5 times prevalence in head and neck cancers. Proteolytically cleaved at the tripeptide Xaa-Pro-Gln, where Xaa in the P(3) position is mostly lysine. The endoprotease may be of microbial origin. Pyroglutamate formation occurs on terminal Gln residues of cleaved peptides. Besides on the N-terminal of mature PBR1, pyroglutamate formation found on at least Gln-58.
Protein Name Basic Salivary Proline-Rich Protein 1
Salivary Proline-Rich Protein Cleaved Into - Proline-Rich Peptide Ii-2 - Basic Peptide Ib-6 - Peptide P-H
Cellular Localisation Secreted
Alternative CLIA Names Basic Salivary Proline-Rich Protein 1 CLIA kit
Salivary Proline-Rich Protein Cleaved Into - Proline-Rich Peptide Ii-2 - Basic Peptide Ib-6 - Peptide P-H CLIA kit
PRB1 CLIA kit
Specificity This kit recognizes Human PRB1 in samples. No significant cross-reactivity or interference between Human PRB1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 92
EDTA plasma(n=8) 98-114 107
Cell culture media(n=8) 85-97 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 91.27 195.41 972.04 88.44 178.36 973.83
Standard deviation 10.40 14.03 90.79 8.55 16.80 97.97
CV (%) 11.39 7.18 9.34 9.67 9.42 10.06
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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