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  • 产品名称：Human MLH1 (MutL Homolog 1) Sandwich ELISA Kit Ready-To-Use (STJE0017015)

  • 产品型号：STJE0017015
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human MLH1 (MutL Homolog 1) Sandwich ELISA Kit Ready-To-Use (STJE0017015)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.057ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This MLH1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: MLH1
Gene ID: 4292
Uniprot ID: MLH1_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.
Post Translational Modifications
Function Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Protein Name Dna Mismatch Repair Protein Mlh1
Mutl Protein Homolog 1
Database Links Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5545483
Reactome: R-HSA-5632987
Reactome: R-HSA-6796648
Reactome: R-HSA-912446
Cellular Localisation Nucleus
Chromosome
Recruited To Chromatin In A Mcm9-Dependent Manner
Alternative ELISA Names Dna Mismatch Repair Protein Mlh1 ELISA kit
Mutl Protein Homolog 1 ELISA kit
MLH1 ELISA kit
COCA2 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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