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  • 产品名称：Human MMP2 (72 kDa type IV collagenase) Sandwich ELISA (STJE0002977)

  • 产品型号：STJE0002977
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human MMP2 (72 kDa type IV collagenase) Sandwich ELISA (STJE0002977)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This MMP2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: MMP2
Gene ID: 4313
Uniprot ID: MMP2_HUMAN
Specificity: This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro. The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
Function Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Protein Name 72 Kda Type Iv Collagenase
72 Kda Gelatinase
Gelatinase A
Matrix Metalloproteinase-2
Mmp-2
Tbe-1 Cleaved Into - Pex
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-381426
Reactome: R-HSA-3928665
Reactome: R-HSA-6785807
Reactome: R-HSA-9009391
Cellular Localisation Isoform 1: Secreted
Extracellular Space
Extracellular Matrix
Membrane
Nucleus
Colocalizes With Integrin Alphav/Beta3 At The Membrane Surface In Angiogenic Blood Vessels And Melanomas
Found In Mitochondria
Along Microfibrils
And In Nuclei Of Cardiomyocytes
Isoform 2: Cytoplasm
Mitochondrion
Alternative ELISA Names 72 Kda Type Iv Collagenase ELISA kit
72 Kda Gelatinase ELISA kit
Gelatinase A ELISA kit
Matrix Metalloproteinase-2 ELISA kit
Mmp-2 ELISA kit
Tbe-1 Cleaved Into - Pex ELISA kit
MMP2 ELISA kit
CLG4A ELISA kit
Specificity This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 92
EDTA plasma (n=8) 87-99 92
Cell culture media (n=8) 88-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.40 5.30 21.80 2.40 5.10 20.80
Standard deviation 0.10 0.30 0.90 0.20 0.20 0.70
CV (%) 4.17 5.66 4.13 8.33 3.92 3.37
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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