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  • 产品名称：Human PPY (Pancreatic prohormone) Sandwich ELISA (STJE0003549)

  • 产品型号：STJE0003549
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human PPY (Pancreatic prohormone) Sandwich ELISA (STJE0003549)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 28.13pg/mL
Detection Limit: 46.88~3000pg/mL
Short Description: This PPY Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PPY
Gene ID: 5539
Uniprot ID: PAHO_HUMAN
Specificity: This kit recognizes Human PP in samples. No significant cross-reactivity or interference between Human PP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Pancreatic Polypeptide Prohormone
Ph
Pancreatic Polypeptide Y
Obinepitide Cleaved Into - Pancreatic Polypeptide
Hpp
Pp - Pancreatic Icosapeptide
Pi
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative ELISA Names Pancreatic Polypeptide Prohormone ELISA kit
Ph ELISA kit
Pancreatic Polypeptide Y ELISA kit
Obinepitide Cleaved Into - Pancreatic Polypeptide ELISA kit
Hpp ELISA kit
Pp - Pancreatic Icosapeptide ELISA kit
Pi ELISA kit
PPY ELISA kit
PNP ELISA kit
Specificity This kit recognizes Human PP in samples. No significant cross-reactivity or interference between Human PP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-106 99
EDTA plasma (n=8) 84-97 91
Cell culture media (n=8) 91-108 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 165.61 471.01 1376.52 168.73 468.55 1479.39
Standard deviation 9.34 23.36 72.13 11.44 21.83 66.13
CV (%) 5.64 4.96 5.24 6.78 4.66 4.47
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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