产品详情
简单介绍：
Human RUNX2 (Runt-related transcription factor 2) Sandwich ELISA (STJE0002592)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.19ng/mL |
| Detection Limit: | 0.31~20ng/mL |
| Short Description: | This RUNX2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | RUNX2 |
| Gene ID: | 860 |
| Uniprot ID: | RUNX2_HUMAN |
| Specificity: | This kit recognizes Human RUNX2 in samples. No significant cross-reactivity or interference between Human RUNX2 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated.probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation. Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340. |
| Function | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. |
| Protein Name | Runt-Related Transcription Factor 2 Acute Myeloid Leukemia 3 Protein Core-Binding Factor Subunit Alpha-1 Cbf-Alpha-1 Oncogene Aml-3 Osteoblast-Specific Transcription Factor 2 Osf-2 Polyomavirus Enhancer-Binding Protein 2 Alpha A Subunit Pea2-Alpha A Pebp2-Alpha A Sl3-3 Enhancer Factor 1 Alpha A Subunit Sl3/Akv Core-Binding Factor Alpha A Subunit |
| Database Links | Reactome: R-HSA-2032785 Reactome: R-HSA-8878166 Reactome: R-HSA-8939246 Reactome: R-HSA-8939902 Reactome: R-HSA-8940973 Reactome: R-HSA-8941284 Reactome: R-HSA-8941326 Reactome: R-HSA-8941332 Reactome: R-HSA-8941333 |
| Cellular Localisation | Nucleus Cytoplasm |
| Alternative ELISA Names | Runt-Related Transcription Factor 2 ELISA kit Acute Myeloid Leukemia 3 Protein ELISA kit Core-Binding Factor Subunit Alpha-1 ELISA kit Cbf-Alpha-1 ELISA kit Oncogene Aml-3 ELISA kit Osteoblast-Specific Transcription Factor 2 ELISA kit Osf-2 ELISA kit Polyomavirus Enhancer-Binding Protein 2 Alpha A Subunit ELISA kit Pea2-Alpha A ELISA kit Pebp2-Alpha A ELISA kit Sl3-3 Enhancer Factor 1 Alpha A Subunit ELISA kit Sl3/Akv Core-Binding Factor Alpha A Subunit ELISA kit RUNX2 ELISA kit AML3 ELISA kit CBFA1 ELISA kit OSF2 ELISA kit PEBP2A ELISA kit |
| Specificity | This kit recognizes Human RUNX2 in samples. No significant cross-reactivity or interference between Human RUNX2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 91-102 | 96 |
| EDTA plasma (n=8) | 87-102 | 94 |
| Cell culture media (n=8) | 93-111 | 101 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.04 | 2.52 | 6.92 | 1.09 | 2.50 | 6.44 |
| Standard deviation | 0.06 | 0.14 | 0.32 | 0.07 | 0.12 | 0.26 |
| CV (%) | 5.77 | 5.56 | 4.62 | 6.42 | 4.80 | 4.04 |
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