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  • 产品名称：Human SDC4 (Syndecan-4) CLIA Kit (STJC0000753)

  • 产品型号：STJC0000753
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human SDC4 (Syndecan-4) CLIA Kit (STJC0000753)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This human SDC4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: SDC4
Gene ID: 6385
Uniprot ID: SDC4_HUMAN
Specificity: This kit recognizes Human SDC4 in samples. No significant cross-reactivity or interference between Human SDC4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Detected in fibroblasts (at protein level). Also expressed in epithelial cells.
Post Translational Modifications Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. O-glycosylated.contains both chondroitin sulfate and heparan sulfate. Ser-39, Ser-61 and Ser-63 can all be modified by either chondroitin sulfate or heparan sulfate, and the protein exists in forms that contain only chondroitin sulfate, only heparan sulfate and both chondroitin sulfate and heparan sulfate.
Function Cell surface proteoglycan which regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Protein Name Syndecan-4
Synd4
Amphiglycan
Ryudocan Core Protein
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022928
Reactome: R-HSA-2024096
Reactome: R-HSA-202733
Reactome: R-HSA-3000170
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Reactome: R-HSA-4420332
Reactome: R-HSA-9694614
Reactome: R-HSA-975634
Cellular Localisation Isoform 1: Membrane
Single-Pass Type I Membrane Protein
Secreted
Shedding Of The Ectodomain Produces A Soluble Form
Isoform 2: Secreted
Alternative CLIA Names Syndecan-4 CLIA kit
Synd4 CLIA kit
Amphiglycan CLIA kit
Ryudocan Core Protein CLIA kit
SDC4 CLIA kit
Specificity This kit recognizes Human SDC4 in samples. No significant cross-reactivity or interference between Human SDC4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-109 103
EDTA plasma(n=8) 100-115 107
Cell culture media(n=8) 90-101 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.98 2.42 8.32 0.97 2.20 9.03
Standard deviation 0.11 0.24 0.71 0.12 0.18 0.73
CV (%) 11.22 9.92 8.53 12.37 8.18 8.08
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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