产品详情
简单介绍：
Human SEMA7A (Semaphorin-7A) CLIA Kit (STJC0000512)
详情介绍：
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This human SEMA7A kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | SEMA7A |
| Gene ID: | 8482 |
| Uniprot ID: | SEM7A_HUMAN |
| Specificity: | This kit recognizes Human SEMA7A in samples. No significant cross-reactivity or interference between Human SEMA7A and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. |
| Function | Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. |
| Protein Name | Semaphorin-7a Cdw108 Jmh Blood Group Antigen John-Milton-Hargen Human Blood Group Ag Semaphorin-K1 Sema K1 Semaphorin-L Sema L Cd Antigen Cd108 |
| Database Links | Reactome: R-HSA-416700 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Gpi-Anchor Extracellular Side Detected In A Punctate Pattern On The Cell Membrane Of Basal And Supra-Basal Skin Keratinocytes |
| Alternative CLIA Names | Semaphorin-7a CLIA kit Cdw108 CLIA kit Jmh Blood Group Antigen CLIA kit John-Milton-Hargen Human Blood Group Ag CLIA kit Semaphorin-K1 CLIA kit Sema K1 CLIA kit Semaphorin-L CLIA kit Sema L CLIA kit Cd Antigen Cd108 CLIA kit SEMA7A CLIA kit CD108 CLIA kit SEMAL CLIA kit |
| Specificity | This kit recognizes Human SEMA7A in samples. No significant cross-reactivity or interference between Human SEMA7A and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Serum(n=8) | 96-113 | 103 |
| EDTA plasma(n=8) | 100-114 | 107 |
| Cell culture media(n=8) | 95-108 | 101 |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 99.72 | 188.85 | 770.92 | 98.46 | 176.95 | 794.75 |
| Standard deviation | 8.14 | 20.45 | 52.50 | 11.03 | 14.24 | 78.28 |
| CV (%) | 8.16 | 10.83 | 6.81 | 11.20 | 8.05 | 9.85 |
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