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  • 产品名称：Human SERPINA6 (Corticosteroid-binding globulin) Sandwich ELISA (STJE0003736)

  • 产品型号：STJE0003736
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human SERPINA6 (Corticosteroid-binding globulin) Sandwich ELISA (STJE0003736)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 3.75ng/mL
Detection Limit: 6.25~400ng/mL
Short Description: This SERPINA6 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: SERPINA6
Gene ID: 866
Uniprot ID: CBG_HUMAN
Specificity: This kit recognizes Human CBG in samples. No significant cross-reactivity or interference between Human CBG and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated.binds 5 oligosaccharide chains. Glycosylation in position Asn-260 is needed for steroid binding.
Function Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
Protein Name Corticosteroid-Binding Globulin
Cbg
Serpin A6
Transcortin
Database Links Reactome: R-HSA-194002
Reactome: R-HSA-9757110
Cellular Localisation Secreted
Alternative ELISA Names Corticosteroid-Binding Globulin ELISA kit
Cbg ELISA kit
Serpin A6 ELISA kit
Transcortin ELISA kit
SERPINA6 ELISA kit
CBG ELISA kit
Specificity This kit recognizes Human CBG in samples. No significant cross-reactivity or interference between Human CBG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-102 93
EDTA plasma (n=8) 89-101 95
Cell culture media (n=8) 88-103 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 21.53 40.48 142.30 22.23 39.21 156.49
Standard deviation 1.32 1.91 6.40 1.30 1.91 6.78
CV (%) 6.13 4.72 4.50 5.85 4.87 4.33
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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