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  • 产品名称：Human SHBG (Sex hormone-binding globulin) CLIA Kit (STJC0000897)

  • 产品型号：STJC0000897
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human SHBG (Sex hormone-binding globulin) CLIA Kit (STJC0000897)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.75pmol/mL
Detection Limit: 1.25~80pmol/mL
Short Description: This human SHBG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: SHBG
Gene ID: 6462
Uniprot ID: SHBG_HUMAN
Specificity: This kit recognizes Human SHBG in samples. No significant cross-reactivity or interference between Human SHBG and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Isoform 1 and isoform 2 are present in liver and testis.
Post Translational Modifications Variant Asn-356 contains one N-linked (GlcNAc..) at position 356.
Function Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Protein Name Sex Hormone-Binding Globulin
Shbg
Sex Steroid-Binding Protein
Sbp
Testis-Specific Androgen-Binding Protein
Abp
Testosterone-Estradiol-Binding Globulin
Tebg
Testosterone-Estrogen-Binding Globulin
Cellular Localisation Secreted
In Testis
It Is Synthesized By The Sertoli Cells
Secreted Into The Lumen Of The Seminiferous Tubule And Transported To The Epididymis
Alternative CLIA Names Sex Hormone-Binding Globulin CLIA kit
Shbg CLIA kit
Sex Steroid-Binding Protein CLIA kit
Sbp CLIA kit
Testis-Specific Androgen-Binding Protein CLIA kit
Abp CLIA kit
Testosterone-Estradiol-Binding Globulin CLIA kit
Tebg CLIA kit
Testosterone-Estrogen-Binding Globulin CLIA kit
SHBG CLIA kit
Specificity This kit recognizes Human SHBG in samples. No significant cross-reactivity or interference between Human SHBG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-107 100
EDTA plasma(n=8) 86-101 93
Cell culture media(n=8) 97-113 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (nmol/L) 4.20 11.68 28.66 3.97 12.50 29.93
Standard deviation 0.53 0.92 3.10 0.36 1.05 2.94
CV (%) 12.62 7.88 10.82 9.07 8.40 9.82
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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