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  • 产品名称：Human SPARC (SPARC) CLIA Kit (STJC0000619)

  • 产品型号：STJC0000619
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human SPARC (SPARC) CLIA Kit (STJC0000619)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human SPARC kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: SPARC
Gene ID: 6678
Uniprot ID: SPRC_HUMAN
Specificity: This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites.an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Protein Name Sparc
Basement-Membrane Protein 40
Bm-40
Osteonectin
On
Secreted Protein Acidic And Rich In Cysteine
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1251985
Reactome: R-HSA-3000178
Reactome: R-HSA-3000497
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
In Or Around The Basement Membrane
Alternative CLIA Names Sparc CLIA kit
Basement-Membrane Protein 40 CLIA kit
Bm-40 CLIA kit
Osteonectin CLIA kit
On CLIA kit
Secreted Protein Acidic And Rich In Cysteine CLIA kit
SPARC CLIA kit
ON CLIA kit
Specificity This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 95
EDTA plasma(n=8) 97-110 104
Cell culture media(n=8) 93-108 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 199.57 606.36 1519.01 187.20 654.78 1437.17
Standard deviation 23.89 61.97 136.86 17.73 62.20 99.45
CV (%) 11.97 10.22 9.01 9.47 9.50 6.92
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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