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  • 产品名称：Human sSELL (Soluble L-Selectin) Sandwich ELISA (STJE0019891)

  • 产品型号：STJE0019891
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human sSELL (Soluble L-Selectin) Sandwich ELISA (STJE0019891)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 35.9 pg/mL
Detection Limit: 125-8000 pg/mL
Short Description: This sSELL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: SELL
Gene ID: 6402
Uniprot ID: LYAM1_HUMAN
Specificity: This kit recognizes Human sSELL in samples. No significant cross-reactivity or interference between Human sSELL and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Expressed in B-cell lines and T-lymphocytes.
Post Translational Modifications N-glycosylated.
Function Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Protein Name L-Selectin
Cd62 Antigen-Like Family Member L
Leukocyte Adhesion Molecule 1
Lam-1
Leukocyte Surface Antigen Leu-8
Leukocyte-Endothelial Cell Adhesion Molecule 1
Lecam1
Lymph Node Homing Receptor
Tq1
Gp90-Mel
Cd Antigen Cd62l
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202733
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names L-Selectin ELISA kit
Cd62 Antigen-Like Family Member L ELISA kit
Leukocyte Adhesion Molecule 1 ELISA kit
Lam-1 ELISA kit
Leukocyte Surface Antigen Leu-8 ELISA kit
Leukocyte-Endothelial Cell Adhesion Molecule 1 ELISA kit
Lecam1 ELISA kit
Lymph Node Homing Receptor ELISA kit
Tq1 ELISA kit
Gp90-Mel ELISA kit
Cd Antigen Cd62l ELISA kit
SELL ELISA kit
LNHR ELISA kit
LYAM1 ELISA kit
Specificity This kit recognizes Human sSELL in samples. No significant cross-reactivity or interference between Human sSELL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 99
EDTA plasma (n=8) 88-102 95
Cell culture media (n=8) 95-109 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 428.81 932.30 3713.87 458.72 1018.42 4073.67
Standard deviation 24.40 41.30 188.66 23.95 56.73 148.69
CV (%) 5.69 4.43 5.08 5.22 5.57 3.65
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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