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  • 产品名称：Human SST (Somatostatin) Competitive ELISA (STJE0003327)

  • 产品型号：STJE0003327
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human SST (Somatostatin) Competitive ELISA (STJE0003327)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This SST Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: SST
Gene ID: 6750
Uniprot ID: SMS_HUMAN
Specificity: This kit recognizes Human SST in samples. No significant cross-reactivity or interference between Human SST and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Somatostatin-14: Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH.the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin. Neuronostatin: May enhance low-glucose-induced glucagon release by pancreatic alpha cells. This effect may be mediated by binding to GPR107 and PKA activation. May regulate cardiac contractile function. May compromise cardiomyocyte viability. In the central nervous system, may impair memory retention and may affect hippocampal excitability. May also have anxiolytic and anorexigenic effects. May play a role in arterial pressure regulation. May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH.
Protein Name Somatostatin
Growth Hormone Release-Inhibiting Factor Cleaved Into - Somatostatin-28 - Somatostatin-14
Sst-14 - Neuronostatin
Nst
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-9022702
Cellular Localisation Secreted
Alternative ELISA Names Somatostatin ELISA kit
Growth Hormone Release-Inhibiting Factor Cleaved Into - Somatostatin-28 - Somatostatin-14 ELISA kit
Sst-14 - Neuronostatin ELISA kit
Nst ELISA kit
SST ELISA kit
Specificity This kit recognizes SST in samples. No significant cross-reactivity or interference between SST and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 96
EDTA plasma(n=8) 87-99 92
Cell culture media(n=8) 89-100 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 23.20 66.90 217.40 21.40 63.20 228.60
Standard deviation 1.60 4.00 10.00 1.20 2.70 11.20
CV (%) 6.90 5.98 4.60 5.61 4.27 4.90
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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