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  • 产品名称：Human TBP2 (Thioredoxin Binding Protein 2) Sandwich ELISA (STJE0003193)

  • 产品型号：STJE0003193
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TBP2 (Thioredoxin Binding Protein 2) Sandwich ELISA (STJE0003193)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This TBP2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TXNIP
Gene ID: 10628
Uniprot ID: TXNIP_HUMAN
Specificity: This kit recognizes Human TBP2 in samples. No significant cross-reactivity or interference between Human TBP2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).
Protein Name Thioredoxin-Interacting Protein
Thioredoxin-Binding Protein 2
Vitamin D3 Up-Regulated Protein 1
Database Links Reactome: R-HSA-844456
Reactome: R-HSA-9617629
Reactome: R-HSA-9660826
Reactome: R-HSA-9707564
Cellular Localisation Cytoplasm
Alternative ELISA Names Thioredoxin-Interacting Protein ELISA kit
Thioredoxin-Binding Protein 2 ELISA kit
Vitamin D3 Up-Regulated Protein 1 ELISA kit
TXNIP ELISA kit
VDUP1 ELISA kit
Specificity This kit recognizes Human TBP2 in samples. No significant cross-reactivity or interference between Human TBP2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-106 97
EDTA plasma (n=8) 94-111 101
Cell culture media (n=8) 89-102 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.46 1.15 4.20 0.44 1.13 3.90
Standard deviation 0.03 0.06 0.17 0.03 0.06 0.16
CV (%) 6.52 5.22 4.05 6.82 5.31 4.10
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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