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  • 产品名称：Human TIMP3 (Metalloproteinase inhibitor 3) CLIA Kit (STJC0000653)

  • 产品型号：STJC0000653
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TIMP3 (Metalloproteinase inhibitor 3) CLIA Kit (STJC0000653)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human TIMP3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TIMP3
Gene ID: 7078
Uniprot ID: TIMP3_HUMAN
Specificity: This kit recognizes Human TIMP-3 in samples. No significant cross-reactivity or interference between Human TIMP-3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Protein Name Metalloproteinase Inhibitor 3
Protein Mig-5
Tissue Inhibitor Of Metalloproteinases 3
Timp-3
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Metalloproteinase Inhibitor 3 CLIA kit
Protein Mig-5 CLIA kit
Tissue Inhibitor Of Metalloproteinases 3 CLIA kit
Timp-3 CLIA kit
TIMP3 CLIA kit
Specificity This kit recognizes Human TIMP-3 in samples. No significant cross-reactivity or interference between Human TIMP-3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-100 91
EDTA plasma(n=8) 87-99 92
Cell culture media(n=8) 97-112 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 53.26 118.42 407.87 48.20 110.76 424.16
Standard deviation 4.37 10.50 39.03 5.25 9.00 47.17
CV (%) 8.21 8.87 9.57 10.89 8.13 11.12
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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