产品详情
简单介绍：
Human TJP1 (Tight junction protein ZO-1) Sandwich ELISA (STJE0003029)
详情介绍：
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.09ng/mL |
| Detection Limit: | 0.16~10ng/mL |
| Short Description: | This TJP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | TJP1 |
| Gene ID: | 7082 |
| Uniprot ID: | ZO1_HUMAN |
| Specificity: | This kit recognizes Human TJP1 in samples. No significant cross-reactivity or interference between Human TJP1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ. |
| Function | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. |
| Protein Name | Tight Junction Protein Zo-1 Tight Junction Protein 1 Zona Occludens Protein 1 Zonula Occludens Protein 1 |
| Database Links | Reactome: R-HSA-191650 Reactome: R-HSA-2028269 Reactome: R-HSA-351906 Reactome: R-HSA-8935964 Reactome: R-HSA-9705677 |
| Cellular Localisation | Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Cell Junction Tight Junction Gap Junction Cell Projection Podosome Moves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell Contact At Podosomal Sites Is Predominantly Localized In The Ring Structure Surrounding The Actin Core Colocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells |
| Alternative ELISA Names | Tight Junction Protein Zo-1 ELISA kit Tight Junction Protein 1 ELISA kit Zona Occludens Protein 1 ELISA kit Zonula Occludens Protein 1 ELISA kit TJP1 ELISA kit ZO1 ELISA kit |
| Specificity | This kit recognizes Human TJP1 in samples. No significant cross-reactivity or interference between Human TJP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item Specifications Storage
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
Sample Type Range (%) Average Recovery (%)
| Serum(n=8) | 88-100 | 93 |
| EDTA plasma (n=8) | 96-108 | 101 |
| Cell culture media (n=8) | 90-107 | 97 |
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.29 | 4.40 | 0.55 | 1.17 | 4.57 |
| Standard deviation | 0.03 | 0.06 | 0.24 | 0.03 | 0.05 | 0.14 |
| CV (%) | 6.00 | 4.65 | 5.45 | 5.45 | 4.27 | 3.06 |
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