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  • 产品名称：Human TNFSF14 (Tumor Necrosis Factor Ligand Superfamily, Member 14) Sandwich ELISA (STJE0019893)

  • 产品型号：STJE0019893
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TNFSF14 (Tumor Necrosis Factor Ligand Superfamily, Member 14) Sandwich ELISA (STJE0019893)
详情介绍：
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 2.67 pg/mL
Detection Limit: 7.81-500 pg/mL
Short Description: This TNFSF14 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TNFSF14
Gene ID: 8740
Uniprot ID: TNF14_HUMAN
Specificity: This kit recognizes Human TNFSF14 in samples. No significant cross-reactivity or interference between Human TNFSF14 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
Post Translational Modifications N-glycosylated. The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
Function Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM. Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 14
Herpes Virus Entry Mediator Ligand
Hvem-L
Herpesvirus Entry Mediator Ligand
Cd Antigen Cd258 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 14 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 14 - Soluble Form
Database Links Reactome: R-HSA-5668541
Reactome: R-HSA-5669034
Reactome: R-HSA-5676594
Cellular Localisation Tumor Necrosis Factor Ligand Superfamily Member 14
Membrane Form: Cell Membrane
Single-Pass Type Ii Membrane Protein
Soluble Form: Secreted
Isoform 2: Cytoplasm
Alternative ELISA Names Tumor Necrosis Factor Ligand Superfamily Member 14 ELISA kit
Herpes Virus Entry Mediator Ligand ELISA kit
Hvem-L ELISA kit
Herpesvirus Entry Mediator Ligand ELISA kit
Cd Antigen Cd258 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 14 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 14 - Soluble Form ELISA kit
TNFSF14 ELISA kit
HVEML ELISA kit
LIGHT ELISA kit
UNQ391 ELISA kit
PRO726 ELISA kit
Specificity This kit recognizes Human TNFSF14 in samples. No significant cross-reactivity or interference between Human TNFSF14 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 98
EDTA plasma (n=8) 88-103 94
Cell culture media (n=8) 91-107 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 23.75 76.39 187.63 25.33 75.76 195.79
Standard deviation 1.61 4.51 8.46 1.51 3.11 9.30
CV (%) 6.79 5.91 4.51 5.96 4.11 4.75
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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