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  • 产品名称：Human TXN (Thioredoxin) CLIA Kit (STJC0000764)

  • 产品型号：STJC0000764
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TXN (Thioredoxin) CLIA Kit (STJC0000764)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human TXN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TXN
Gene ID: 7295
Uniprot ID: THIO_HUMAN
Specificity: This kit recognizes Human Trx in samples. No significant cross-reactivity or interference between Human Trx and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
Function Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Protein Name Thioredoxin
Trx
Atl-Derived Factor
Adf
Surface-Associated Sulphydryl Protein
Sasp
Allergen Hom S Trx
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-3299685
Reactome: R-HSA-499943
Reactome: R-HSA-5628897
Reactome: R-HSA-5676934
Reactome: R-HSA-844456
Reactome: R-HSA-9617629
Reactome: R-HSA-9660826
Cellular Localisation Nucleus
Cytoplasm
Secreted
Translocates From The Cytoplasm Into The Nucleus After Phorbol 12-Myristate 13-Acetate Induction (Pma)
Predominantly In The Cytoplasm In Non Irradiated Cells
Radiation Induces Translocation Of Trx From The Cytoplasm To The Nucleus
Secreted By A Leaderless Secretory Pathway
Alternative CLIA Names Thioredoxin CLIA kit
Trx CLIA kit
Atl-Derived Factor CLIA kit
Adf CLIA kit
Surface-Associated Sulphydryl Protein CLIA kit
Sasp CLIA kit
Allergen Hom S Trx CLIA kit
TXN CLIA kit
TRDX CLIA kit
TRX CLIA kit
TRX1 CLIA kit
Specificity This kit recognizes Human Trx in samples. No significant cross-reactivity or interference between Human Trx and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-116 106
EDTA plasma(n=8) 97-109 103
Cell culture media(n=8) 94-110 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 196.20 550.39 1356.34 201.82 506.12 1447.54
Standard deviation 18.60 40.95 115.70 21.31 55.88 91.92
CV (%) 9.48 7.44 8.53 10.56 11.04 6.35
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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