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  • 产品名称：Human TYR (Tyrosinase) CLIA Kit (STJC0000747)

  • 产品型号：STJC0000747
  • 产品厂商：ST john's laboratory
  • 产品文档：
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简单介绍：
Human TYR (Tyrosinase) CLIA Kit (STJC0000747)
详情介绍：
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This human TYR kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TYR
Gene ID: 7299
Uniprot ID: TYRO_HUMAN
Specificity: This kit recognizes Human TYR in samples. No significant cross-reactivity or interference between Human TYR and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Glycosylated.
Function This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
Protein Name Tyrosinase
Lb24-Ab
Monophenol Monooxygenase
Sk29-Ab
Tumor Rejection Antigen Ab
Database Links Reactome: R-HSA-5662702
Cellular Localisation Melanosome Membrane
Single-Pass Type I Membrane Protein
Melanosome
Proper Trafficking To Melanosome Is Regulated By Sgsm2
Ankrd27
Rab9a
Rab32 And Rab38
Alternative CLIA Names Tyrosinase CLIA kit
Lb24-Ab CLIA kit
Monophenol Monooxygenase CLIA kit
Sk29-Ab CLIA kit
Tumor Rejection Antigen Ab CLIA kit
TYR CLIA kit
Specificity This kit recognizes Human TYR in samples. No significant cross-reactivity or interference between Human TYR and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-113 103
EDTA plasma(n=8) 85-98 92
Cell culture media(n=8) 96-113 104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.52 1.33 3.90 0.52 1.34 4.09
Standard deviation 0.07 0.10 0.31 0.06 0.10 0.27
CV (%) 13.46 7.52 7.95 11.54 7.46 6.60
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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